Applied research into the production process of gama - Globulin from Human Plasma and Standard gama - Globulin with Rich of Anti \u2013 HBs
- Author:
Phan Trung Do
;
Duong Tuan Pham
;
Hien Thi Do
;
Thuy Thi Tran
;
Thin Duy Ngo
;
Phuc Hanh Hoang
;
Hai Xuan Le
;
Tri Anh Nguyen
- Publication Type:Journal Article
- Keywords:
Human plasma;
gama - globulin - anti - HBs;
Precipitation of ethanol;
pH and low temperature
- MeSH:
Human plasma;
gama - globulin - anti - HBs;
Precipitation of ethanol;
pH and low temperature
- From:Journal of Medical Research
2008;0(1):67-71
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Introduction: The need for gama \u2013 globulin, especially gama - globulin - anti \u2013 HBs, is huge in Vietnam. A number of patients cannot to afford use them due to the high price as they are imported. Meanwhile, Vietnam has high quality input sources for producing gama \u2013 globulin. \r\n', u'Objectives: To study the production process of gama - Globulin from Human Plasma and Standard gama - Globulin with Rich of Anti \u2013 HBs. \r\n', u'Subjects and method: 168 samples of human plasma from voluntary blood donors, which had been screened with for transfusion transmittable infections (TTLs), were chosen as plasma with rich of anti - HBs. The plasma with anti - HBsAg was precipitated with ethanol 25%, pH 6,9 to gain gama - globulin with rich anti - HBs, which was dried by Dutch Ly - 3 - TTE machine. Activation of anti - HBs gama - globulin was identified by a standard method of the degree of antibody specific for anti - HBsAg kit of BIORAD, \r\n', u'Results: The purity of the gama - globulin achieved was 93%, which was almost equal with the results of some foreign researchers (7.8), the activation of anti - HBs was 1:128 dilution degree. The productivity of gama - globulin gaining from 1 litter plasma was 6.0 gram. This new issue was first demonstrated in Vietnam. \r\n', u'Conclusion: We can domestically produce anti-Bs gama - globulin with high degree of activeness (1:128) from human plasma by the precipitating method with ethanol, pH and low temperature. \r\n', u'
