Study of Methods to Isolate Viable but Non-culturable Microorganisms from Natural Environments
- VernacularTitle:自然界中难分离培养微生物的分离和应用
- Author:
Xiu-Juan YUE
;
Li-Yan YU
;
Qiu-Ping LI
;
Yu-Zhen WEI
;
Yan GUAN
;
Yue-Qin ZHANG
;
- Publication Type:Journal Article
- Keywords:
Viable but non-culturable, Microcolony, Microbial medicine
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
This project is targeted on exploring some improving approaches to isolate and culture the microorganisms which are difficult to be isolated and cultured through the conventional ways. The results showed that betaine, sodium pyruvate, SOD and catalase are helpful for increasing the total number and variety of isolated strains. A kind of combined method was also used to isolate the micro-colony which can not be seen by naked eyes on the plates. Totally 52 Actinomycetes and 103 bacteria and 17 fungi were obtained from 4 soil samples using the above methods. 4. 325% microorganisms were obtained as positive strains to inhibit the growth of some kinds of test bacteria, which is higher than the percent using generally isolated ones. These microbial natural products may remain an important resource for the drug discovery.
