Construction of Recombinant Yeast Converting Xylose Angd Glucose to Ethanol
- VernacularTitle:代谢木糖和葡萄糖的重组酿酒酵母的构建
- Author:
Zhen-Hong YUAN
;
Ya-Ping PAN
;
Ji-Kai LIU
;
Yong-Jie YAN
;
Xiu-Shan YANG
;
- Publication Type:Journal Article
- Keywords:
Recombinant Saccaromyces cerevisiae, xyl1 gene, xyl2 gene, Xylose, Glucose, Ethanol
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Candida shehatae xyl1 gene and Pichia stipitis xyl2 gene were amplified by PCR and the xyl1 and xyl2 were both placed under the promoter GAL of vector pYES2 to produce the recombinant expression vector pYES2-P12. Subsequently the pYES2-P12 vector was transformed into S. cerevisiae YS58 by LiAc to produce the recombinant yeast YSS8-12. It was indicate that the recombinant yeast YSS8-12 could converse xylose to ethanol with the xylose consumption rate of 81. 3%.
