Screening of High Daptomycin-producing Strain by He-Ne Laser Irradiation and Streptomycin Resistance Screening Method
- VernacularTitle:激光诱变玫瑰孢链霉菌结合链霉素抗性筛选法选育达托霉素高产菌株
- Author:
Wen-Yu LU
;
Jian-Ping WEN
;
Jing-Hua FAN
;
Bo-Xiang CAO
;
Bing SUN
;
- Publication Type:Journal Article
- Keywords:
Streptomyces roseosporus, Streptomycin resistance, Daptomycin, He-Ne laser
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The spores suspension of Streptomyces roseosporus D-38 irritated with 20mW He-Ne laser for 20 min were incubated on G1 medium plates containing 1. 9?g/mL of streptomycin. Ten percent of mutants increased the potency of daptomycin by streptomycin-resistance method, including the mutant LC-54, which could produce daptomycin 81. 2 mg/L, which was 39% higher than that of the beginning strain by flask fermentation.
