High-sensitive Detection Method for Siderophores from Pseudomonas
- VernacularTitle:高灵敏假单胞菌铁载体的平板检测方法
- Author:
Shao-Xing CHEN
;
Xiang ZHAO
;
Ping SHEN
;
Zhi-Xiong XIE
;
- Publication Type:Journal Article
- Keywords:
Siderophore, CAS assay, Pseudomonas, MSA medium
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
CAS (Chrome azurol S) assay was a universal method for detecting the bacterial siderophores, and Sugar-Asn liquid medium has been applied to the studies on siderophores from Pieudomonas. In this paper, Asp has been substituted for Asn, and MSA-CAS agar plate was developed by integrating the MSA (sugar-Asp) medium and CAS bright blue dye, which has been used in the universal CAS assay. On the aspect of siderophores detection , 8 strains of 7 species from Pseudomonas had been screened on MSA-CAS agar plates and universal CAS assay respectively. The results showed that MSA-CAS agar was higher-sensitive and lower basic fluorescent than universal CAS assay.
