The Techniques of Isolation and Determination of Ergosterol as the Indicator of Fungal Biomass
- VernacularTitle:真菌生物量指示剂麦角固醇的分离及测定方法
- Author:
Xing-Mei XI
;
Guang-Ming ZENG
;
Hong-Yan YU
;
Jian-Bing LI
;
Guo-He HUANG
;
- Publication Type:Journal Article
- Keywords:
Fungi, Biomass, Ergosterol, Compost
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Ergosterol is the important component of the fungal membrane, and having stable structure. This makes it a suitable indicator for growth of fungi. In the paper, isolation and determination techniques of ergosterol as the indicator of the fungal biomass were reviewed. The methods of extracting ergosterol include traditional saponification and refluxing, rapid physical disruption, rapid ultrasonication, supercritical fluid extraction and so on. The ergosterol determination methods are high performance liquid chromatography, gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry, and thin-layer chromatography, et al. The application of these techniques was also introduced. Finally, the paper prospected the feasibility of applying the ergosterol as the indicator of fungal biomass in composting.
