Selection of bFGF Mimic Peptide by Phage Display
- VernacularTitle:噬菌体展示技术筛选bFGF模拟短肽
- Author:
Hui-Xian HUANG
;
Xiao-Ping WU
;
Shao-Hui CAI
;
Xiao-Kun LI
;
- Publication Type:Journal Article
- Keywords:
Basic fibroblast growth factor Fibroblast growth factor receptor Phage display Heptapeptide
- From:
China Biotechnology
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To obtain the bFGF mimic peptide binding to FGFR via phage display, and to provide the base for developing peptide agonist of bFGF. Methods: Using Balb/c 3T3 cells as the target cells and COS-7 cells as the subtractive panning, the phage display heptapeptide library was biopanned for 4 rounds to obtain the single phage clones. The affinity and the specificity of the clones were assessed by ELISA. DNA sequencing was applied to further analyze the positive clones. Results: Twelve positive clones were selected from the enriched phages. A group of hydrophobic peptides containing a conserved motif, PR, was identified. Conclusion: Two bFGF mimic heptapeptides binding to FGFR were selected, which may be used as the candidates for bFGF agonist.
