Effect of rhBMP-2 on the Osteogenesis of Osteoblast Compounded Chitosan
- VernacularTitle:rhBMP-2对壳聚糖复合成骨细胞后的成骨活性影响
- Author:
Ai-Jun ZUO
;
Dong-Chun LIANG
;
Bao-Li WANG
;
Gang GUO
;
Jing-Yu ZHANG
;
- Publication Type:Journal Article
- Keywords:
rhBMP-2 Ccomplex Tissue-engineering Bone formation
- From:
China Biotechnology
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Recombinant human BMP-2 was compounded with chitosan/gelatin/hydroxyapatite(HCG) scaffold and the complex was sterilized by 60Co radiating. Osteoblast isolated from cranial bones of newborn rat was primary cultured and seeded onto the complexes. 3 days after culturing, scanning electron microscope(SEM) was applied to detect the compatibility of the cell with the complex. SEM showed osteoblast attached closely with the complex and grew well in its pores. Then the complexes with osteoblast modification were implanted into athymic nude mice subcutaneously. 8 weeks after implantation, X-ray photograph and histological observation were applied to detect the bone formation of the complexes. Under X-ray a high-density areas consistent with the shape of the implanted complex could be seen. Histological observation also proved there was bone formation in the interspace of the complex. A conclusion was drawn that rhBMP-2 compounded HCG scaffold had good osteogenesis ability in vivo.
