Gene Coloning, Expression and Activity Assay of Survivin
- VernacularTitle:Survivin的表达和功能研究
- Author:
Yu-Hua LIAO
;
Xiu-Fei YIN
;
- Publication Type:Journal Article
- Keywords:
Survivin Expression Activity assay
- From:
China Biotechnology
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Survivin expresssion in embryo spleen, embryo kidney, embryo liver and in many cancer tissues was determined by RT-PCR, while not in the health liver tissue. Construct the engineered Escherischia coli expressing human survivin and identify the expressed human survivin by Westen-blot. The combination activity of Survivin and RhSmac was determined in vitro. L929 cells transferred with Survivin can survive longer than which transferred with BSA.
