Multidrug-resistant Clinical Enterobacter cloacae Isolates: Analysis of Their ESBLs Gene Types and Molecular Epidemiology
- VernacularTitle:多重耐药阴沟肠杆菌临床分离株ESBLs基因型别和分子流行病学分析
- Author:
Hong LIU
;
Xiao-Fei JIANG
;
Fei-Yi RUAN
;
Min LI
;
Fu-Qi AI
;
Yi-Min MA
;
Xiu-Hua HONG
;
Yuan LV
;
- Publication Type:Journal Article
- Keywords:
Enterobacter cloacae;
beta-lactamases;
PCR
- From:
Chinese Journal of Nosocomiology
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To understand the distribution and epidemiology of ESBLs in ceftazidime or cefotaxime-(resistant) clinical Enterobacter cloacae isolates.METHODS Twenty seven ceftazidime or cefotaxime-resistant(nonrepetitive) E.cloacae were collected from 27 patients hospitalized at the Huashan Hospital,Shanghai.PCR and(sequencing) were performed to understand the distribution of ESBLs in E.cloacae;rep-PCR was(performed) to(understand) the epidemiology of ESBLs in E.cloacae.RESULTS CTX-M-3 like(ESBLs) were the most prevalent in our study(48%);this was the first report of VEB-1-like ESBLs in the member of(Enterobacteriaceae) in China,and the first report of the ESBLs VEB-1-like and CTX-M-3-like in an isolate simultaneously;the majority of(ESBLs) producers exhibited the same rep-PCR pattern,but harbored different ESBLs gene.(CONCLUSIONS) In our study,ESBLs have become prevalent in clinical E.cloacae isolates,and become an important factor of E.cloacae isolates resistant to extended-spectrum beta(-lactams).
