Investigation of Apoptosis of the SGC7901 Cells Induced by the Expression of the Recombinant Gene of anti-HER2 ScFv/tBid
- VernacularTitle:重组抗HER2 ScFv／tBid基因的表达对SGC7901胃癌细胞的促凋亡作用
- Author:
Fang WANG
;
Li-Feng WANG
;
Xiu-Chun QIU
;
Yan-Ming XU
;
Wei BAO
;
Yan-Ling MENG
;
Cheng-Ji WANG
;
Qing-Yu FAN
;
An-Gang YANG
- Publication Type:Journal Article
- Keywords:
Fusion protein of anti-HER2 ScFv/tBid SGC7901 cell Apoptosis
- From:
China Biotechnology
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objetive: To investigate whether apoptosis of SGC7901 cells can be induced by the expression of the recombinant gene of anti-HER2 ScFv/tBid. Methods: The recombinant anti-HER2 ScFv/tBid gene was cloned into vector pCMV and the recombinant plasmid was transfected into SGC7901 cells. The gene expression was detected by RT-PCR and immunofluorescent staining. Cell counting was carried out to show the effect of the gene transfection on cell growth. At the same time, significant apoptotic peak was detected by flow cytometry in recombinant anti-HER2 ScFv/tBid gene transfected cells. Results: The fusion protein of anti-HER2 ScFv/tBid was observed in the cytoplasm of transfected SGC7901 cells. The transfected cells displayed typical cell growth inhibition and apoptosis. Conclusion: Fusion protein of anti-HER2 ScFv/tBid can induce apoptosis of SGC7901.
