Effect of Recombinant N-segment of Thrombospondin-1 on the Proliferation of ECV304
- VernacularTitle:重组凝血酶敏感蛋白-1N端片段对CD36表达阴性的ECV304细胞增殖的抑制
- Author:
Li-Fang WANG
;
Yu-Ling ZHOU
;
Jie GU
;
Zhong-Chao HAN
;
- Publication Type:Journal Article
- Keywords:
TSP-1 Expression and purification ECV304 CD36
- From:
China Biotechnology
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To construct an expression system containing the N-terminal segment of Thrombospondin-1 (TSP-1) in E. coli. To investigate the activity of TSF. Methods: thbs1 gene fragment was amplified from human fetal cord vein endothelial cells and inserted into plasmid pET32c ( + ) which was then transfected and expressed in E. coli. Investigate the effect of recombinant TSF to the proliferation of ECV304 in vitro with MTT. The expression level of CD36 was assayed by FACS. Results: Recombinant TSF was purified. TSF could restrain the proliferation of ECV304 with CD36 low-expression. Conclusions:Low dose of rTSF was a latent assistant treatment of anti-cancer.
