Expressing, Purification and Identification of Neuritin Gene in the E. coli
- VernacularTitle:人Neuritin在原核表达系统的构建及表达纯化
- Author:
Juan TANG
;
Na YU
;
Liang-Sheng WU
;
Lei YANG
;
Ling-Ling XIAN
;
Yan-Hong HUANG
;
Shu-Jun ZHANG
;
Jin HUANG
;
- Publication Type:Journal Article
- Keywords:
Human neuritin Prokaryotic expression cloning Purification
- From:
China Biotechnology
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Neuritin is a new neurotrophic factor found recently. In order to identify the function of Neuritin clearly, the coding sequence of human neuritin was amplified by PCR from neuritin cDNA , this fragment digested by NocI and NotI was inserted into pET32a by T4 ligase and transformed into E. coli BL21 then the recombinant plasmid named pET32a-neuritin was constructed successfully . Neuritin was expressed distinctly after inducing by EPTG. The product was identified as neuritin by SDS-PAGE and Western blot analysis . The expression production was purified on Ni2+-NTA column.
