Research on Isolating Bacteria by Using Magnetophoresis
- VernacularTitle:磁泳分离细菌新方法的研究
- Author:
Xin-Xing LIU
;
Jian-Ping XIE
;
Wen-Bin LIU
;
Qiang HUO
;
Guan-Zhou QIU
;
- Publication Type:Journal Article
- Keywords:
Magnetophoresis Nanoparticles Magnetosome Bacterial isolation Magnetotactic bacteria
- From:
China Biotechnology
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
There are similarities between magnetotactic bacteria and Acidithiobacillus ferrooxidans (A. ferrooxidans) which isolated from Acid mine drainage(AMD). The weak magnetotaxis of some bioleaching bacteria isolated were found by microscope. A magnetophoresis apparatus was designed based on these weak magnetotaxis and be used to analysis the movement of these strains. The physiological properties of the anear magnetic field strain and removed magnetic field strain which isolated successfully by magnetophoresis apparatus have large difference. The nanometer magnetic particles was extract from the Acidithiobacillus ferrooxidans which purified by spread plate method from AMFS and its main elements are Fe and O by energy spectrum analysis. The results show that A. ferrooxidans have weak magnetotaxis and can be isolated by magnetophoresis. With the development of this new isolating method, the research of magnetotactic bacteria and bioleaching will get more benefit from it.
