A Speedy Extraction Method for Bacterial Chromosomal DNA
- VernacularTitle:一种快速提取细菌总DNA的方法研究
- Author:
Wei ZHENG
;
Chun-Shan QUAN
;
Yong-Zhe PIAO
;
Sheng-Di FAN
;
- Publication Type:Journal Article
- Keywords:
Bacterium chromosomal DNA High temperature Digestion 16S rRNA Environmental DNA
- From:
China Biotechnology
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
How to get functional gene from uncultured-microbiology is the hotspot content of microbial ecology. What the most important is how to obtain the pure and integrated genomic DNA. An efficient, nonselective extraction method to gain chromosomal DNA from eight kinds of bacteria was introduced. Amount DNA released by hot-detergent gave the highest DNA yields from different G + and G- bacteria. Running 20 hours by PFGE mode, the size of total DNA is over 23kb. The pure DNA could be digested by Hind Ⅲ and used in PCR. The total environmental DNA also can be extracted from soil by the same method. As a result it showed a new way for the environmental DNA extraction.
