Study on Photolysis of Vitamin B_(12) and New Method to Determine the Contents of Vitamin B_(12) in Fermentation Broth
- VernacularTitle:维生素B_(12)的光解研究及发酵液中维生素B_(12)检测新方法的建立
- Author:
Lei WANG
;
Yu-Ming ZHANG
;
Yun-Shan WANG
;
Li-Ping ZHANG
;
Zhi-Guo SU
;
- Publication Type:Journal Article
- Keywords:
Vitamin B12 Hydroxycobalamin Photolysis Propionibacterium freudenreichii
- From:
China Biotechnology
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The photolytic behavior of deoxyadenosylcobalamin and methylcobalamin in water solution was investigated with high performance liquid chromatography. The results indicated that the photolytic cleavage rate increased with the light intensity, according to which a new method was developed to determine the concentration of vitamin B12 in fermentation broth. The samples were completely photolyzed after cell disruption. The content of vitamin B12 was obtained by determining the content of the hydroxycobalamin. The method shows many advantages, such as rapidness, high accuracy and low sample quantity needed, over traditions methods. The developed method may be used in the field of vitamin B12 fermentation.
