2D-PAGE Analysis of Chinese Rose Leaf Protein Under Heat Shock Stress
- VernacularTitle:热胁迫下月季叶片蛋白双向电泳分析
- Author:
Rui JIANG
;
Yong-Hong HU
;
Chang-Hua JIANG
;
Hong-Wei ZHAO
;
Shang-Lian HU
;
Feng MING
;
- Publication Type:Journal Article
- Keywords:
Chinese rose Heat shock stress 2D-PAGE PMF
- From:
China Biotechnology
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Proteins extracted from two varieties of Chinese roses leaves were separated by two- dimensional polyacrylamide gel electrophoresis (2-DE) with immobilized pH gradient (IPG). Many difference proteins were isolated with molecular weights ranging 10-30 kDa and pI5-6. Three proteins of high levels observed in a gel were excised and identified using peptide mass fingerprinting and MS-MS. A summary of the identified proteins and their putative functions are presented. They are identified as eIF-5A、LEA protein and Hsp17. 5. Functions of these proteins in plant tolerance to high temperature were discussed.
