Study on the Optimal Fermentation Process for Production Chitinase of Streptomyces sp. A048
- VernacularTitle:链霉菌A048产几丁质酶最佳发酵工艺研究
- Author:
Li-You QIU
;
Ming-Dao WANG
;
Yuan-Chen QI
;
Pei-Lin YUAN
;
Xin-Cheng JIA
;
- Publication Type:Journal Article
- Keywords:
Chitinase,Streptomyces,Fermentation process
- From:
Microbiology
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Streptomyces sp. A048 was cultured in a complete medium to the last stage of log phase,the hyphae were washed and collected by centrifugation. Then the hyphae were inoculated in liquid medium for chitinase production using two-step fermentation. Activity of chitinase produced by two-step fermentation was 1.1 times higher than that from one-step fermentation,and ferment cycle was for 54 hours,which was 66 hours shorter than that of one-step fermentation. The hyphae and the powder of chitin were co-immobilizated and cultured in liquid medium for 36 hours,activity of chitinase was 1.8 times higher than that from one-step fermentation,and ferment cycle was 54h shorter than that of one-step fermentation. By adding 0.4% cellulose to two-step fermentation,activity of chitinase was 18.52 U/mL that was 4 times higher than that from the control and 10 times higher than that from one-step fermentation. Two step fermentation with chitin and cellulose may be the optimal fermentation process to produce Chitinase from Streptomyces sp. A048.
