Influence of RNA interference on expression of Aurora-A in human ovarian cancer cells and on cell proliferation
- VernacularTitle:RNA干扰对Aurora-A在人卵巢癌细胞中表达及细胞增殖的影响
- Author:
Su-Rong WANG
;
Guo-Hong LIU
;
Bo WANG
;
- Publication Type:Journal Article
- Keywords:
RNA interference;
Aurora-A;
SKOV3;
ovarain cancer;
apoptosis
- From:
Chinese Journal of Cancer Biotherapy
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the inhibitory effect of RNA interference (RNAi) on the expression of Aurora-A in SKOV3 cells and on proliferation of SKOV3 cells.Methods:Two pairs of oligo small interference RNA (Oligo siRNA) specific for Aurora-A were designed for RNAi and were transferred into SKOV3 cells.The expression of Aurora-A were de- tected by RT-PCR and Western blot.Furthermore,the cell proliferation and apoptosis were observed by MTT and FCM af- ter transfection.Results:After transfection with Oligo siRNA,mRNA and protein level of Aurora-A gene in SKOV3 cells were obviously reduced,while the inhibitory rate of proliferation and apoptosis rate in SKOV3 cells were increased signifi- cantly.Conclusion:The Oligo siRNA specific for Aurora-A can reduce the expression of Aurora-A gene and induce apop- tosis of SKOV3 cells.
