Characteristics of HEK293 Cells Growth and Metabolism under Carrier-free Immobilization Culture Mode
- VernacularTitle:无载体固定化培养模式下HEK293细胞的生长和代谢特征
- Author:
Xing-Mao LIU
;
Hong LIU
;
Ben-Chuan WU
;
Ling-Ling YE
;
Shi-Chong LI
;
Xiao-Ping NI
;
Zhao-Lie CHEN
;
Pei-Tang HUANG
;
- Publication Type:Journal Article
- Keywords:
Immobilization culture HEK293 cells Growth Metabolism
- From:
China Biotechnology
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
By using the cell density, cell viability, size distribution of cell aggregates,specific consumption rate of glucose (q_ glc ), specific production rate of lactate (q_ lac ), lactate transform rate (Y_ lac/glc ) and amino acids utilization as the evaluation indexes, the growth and metabolism of HEK293 cells under carrier-free immobilization culture mode were examined and compared with those of HEK293 cells cultured in static tissue flasks. It was found that HEK293 cells grown as suspended cell aggregates in spinner flasks maintained the basic growth and metabolism characteristics of HEK293 cells in stationary anchored culture, and HEK293 cells under carrier-free immobilization culture mode as suspended aggregates in stirred bioreactor facilitate perfusion performance and increase unit productivity. Cultivation of HEK293 cells in carrier-free immobilization culture mode has potential for further improving mammalian cells culture technique.
