Prokaryotic Expression of the Partial gB Gene of the Marek’s Disease Virus
- VernacularTitle:马立克氏病病毒gB部分基因的原核表达
- Author:
Ya-Feng QIU
;
Fei-Fei GE
;
Pu-Yan CHEN
;
- Publication Type:Journal Article
- Keywords:
Marek’s disease virus Prokaryotic expression Glycoprotein Epitope
- From:
China Biotechnology
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
The partial segment of Marek′s disease virus (MDV) glycoprotein B (gB) gene was amplified by PCR. The segment was cloned into pET-28a vector to obtain the recombinant pET-gB plasmid. The recombinant plasmid was transformed into E.coli BL21,and expressed in very high level as inclusion body after induced with 1.0mmol/L IPTG. The inclusion body was solubilized in urea (8mol/L) . The purified protein was obtained by use of His?Bind affinity chromatography. Mice were immunized i.p. by the purified protein to make the polyclonal antibody. The titer of the antibody by indirect ELISA was 1?10~ -5 . Moreover, the analysis by western blot proved that antibody was specific to the recombinant protein. These works lay a favorable foundation for the study of the immune response by MDV gB.
