Preparation and Identification of Monoclonal Antibody Against Cloxacillin
- VernacularTitle:邻氯青霉素单克隆抗体的制备及特性鉴定
- Author:
Lin WANG
;
Meng-Wen QI
;
Cai-Wei FENG
;
Li-Li MENG
;
- Publication Type:Journal Article
- Keywords:
Betalactam antibiotics Cloxacillin Monoclonal antibody
- From:
China Biotechnology
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
CX-conjugated antigen(BSA-CX) were produced by methods of EDC.The spleen cells of BALB/C mouse immunized by BSA-CX were fused with SP2/0 plasmacytoma cells using PEG4000,and selected cultured with HAT and HT medium.A hybridoma cell line of 2H8-B1-F4 was secreened for specificity to CX,and cloned by limited dilution method for 3 times,which secrete high affinity and stable monoclonal antibodies against CX with indirect ELISA. The antibody was characterized by IgG1 isotype, the titers of antibody in cell supernatant and ascites were 1∶1000 and 1∶4?10~5,respectively.The mAb against CX was selective toward dicloxacillin, oxacillin with cross-reactivity of about 19.6% and 21.3%,which can be used to detect CX residues by competitive ELISA.
