Cloning and Characterization Analysis of 60S Ribosomal Protein L10a Gene from Chaetomium globosum
- VernacularTitle:球毛壳菌60S核糖体蛋白L10a基因克隆与特性分析
- Author:
Zhi-Hua LIU
;
Qian YANG
;
- Publication Type:Journal Article
- Keywords:
Chaetomium globosum,60S ribosomal protein L10a,Gene cloning,Characterization analysis
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The sequence of Neurospora crassa(XP_322380)and Gibberella zeae PH-1(EAA76971)ribosomal protein gene were subjected to local tBlastn searching against the Chaetomium globosum ESTs datebase.The 765 bp full length cDNA encoding 60S ribosomal protein L10a gene was obtained.The open reading frame was 654 bp and encoded 217 amino acids.The protein molecular mass was 23.9 kD.The BlastP analysis revealed that amino acids sequence of ribosomal protein L10a gene from C.globosum shared 89% high similarity with N.crassa and 78% low similarty with Ustilago maydis.The cDNA and deduced amino acid sequence of 60S ribosomal protein L10a gene were accepted by GenBank(accession numbers: AY669070,AAT74578).
