Optimization of Solid State Fermentation of Endo-mannanase Production by Bacillus subtilis
- VernacularTitle:枯草芽孢杆菌产?-甘露聚糖酶固体发酵条件的优化
- Author:
Yun WEI
;
Zheng-Qiang JIANG
;
- Publication Type:Journal Article
- Keywords:
Bacillus subtilis,Wheat bran,Mannanase,Konjac powder
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Bacillus subtilis is a good producer of mannanase.This is the first study to investigate mannanase production under solid state fermentation(SSF) by Bacillus subtilis.Bacillus subtilis WY34 was used for the production of extracellular mannanase in the SSF of wheat bran.The highest mannanase activity was 7,650 U/g dry matter when the following conditions were used: wheat bran 5g,initial moisture content 71%,Tween-80 1%,konjac powder 0.4 g,initial pH 7.0,incubation temperature 50 ℃.Enzyme production was increased 178% after optimization.