Optimal Study on the Fermentation Conditions of S-adenosyl-L-methionine
- VernacularTitle:发酵生产S-腺苷-L-蛋氨酸培养条件的优化研究
- Author:
Han-Zhu DONG
;
Pei-Yi LIU
;
Tian-Wei TAN
;
- Publication Type:Journal Article
- Keywords:
Fermentation,S-adenosyl-L-methionine,Yeast,Uniform design
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
This paper focuses mainly on the study of optimal fermentation conditions of S-adenosyl-L-methionine.Effects of carbon sources,nitrogen sources,inorganic constituents,growth factors and adding time of L-methionine on the yield,the content and biomass of S-adenosyl-L-methionine are studied.And ingredients of the culture medium are also optimized by the method of uniform design.The final optimum culture medium contains: glucose 30 g,Yeast powder 11 g,(NH_4)_2SO_4 12 g,K_2HPO_4?3H_2O 5 g,KH_2PO_4 10 g,MnSO_4?H_2O 0.09 g,ZnSO_4?7H_2O 0.14 g,MgCl_20.5 g,CaCl_2 0.3 g,CuSO_4 0.005 g per liter. Using that optimum culture medium,the yield of S-adenosyl-L-methionine can reach 0.9 g/L in Erlenmeyer flask which is 30 % higher than before.Experiment on 5 L fermenter reveals that the accumulation of S-adenosyl-L-methionine can reach 2.66 g/L.Biomass is 23.4 g/L.
