Pulsed Field Gel Electrophoresis and Its Application in Fungi
- VernacularTitle:脉冲场凝胶电泳技术及其在真菌学研究中的应用
- Author:
Xiao-Yan FU
;
Ke-Xing HU
;
Li FAN
;
- Publication Type:Journal Article
- Keywords:
Pulsed field gel electrophoresis(PFGE),Fungi,Identification of species groups,Genetic analysis
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Pulsed field gel electrophoresis(PFGE)is a new type technique of gel electrophoresis which can be used to separate large DNA molecules.It has been widely applied to the karyotype analysis,identification of species groups,genetic orientation and genetic analysis for fungi.This article describes the principle,development and general manipulative procedure of PFGE,and elaborates the application in the molecular research of fungi.