Selection of High-yield Agro-antibiotic 2-16 Producing Strain and Optimization of Its Cultivation
- VernacularTitle:农用抗生素2-16高产菌株选育及发酵优化组合研究
- Author:
Xiao-Qing WANG
;
Hong-Mei ZENG
;
Yi-Ping SHI
;
- Publication Type:Journal Article
- Keywords:
Agro-antibiotic 2-16,Mutation,SAS software,Medium optimization
- From:
Microbiology
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to improve the production of agro-antibiotic 2-16,the producing strain(Streptomyces ahygroscopicus var.huangshanensis) was treated by protoplast regeneration,ultraviolet radiation,NTG mutagenesis and low energy C~(+) ion implantation.At last,a high-yield strain No.515 was obtained.The production of ~()No.515 was increased by 223.10%.By using Plackett-Burman design and Response Surface Analysis provided by SAS software,the cultivation condition of No.515 was optimized.The amount of agro-antibiotic 2-16 was increased by 38.53% when the strain No.515 was cultivated in the optimum medium instead of the initial one.
