Studies on Establishing and Optimizing Conditions of Concentration Virus in Water Body
- VernacularTitle:水体病毒浓缩方法的建立和优化研究
- Author:
Bao-Jin PAN
;
Jun-Yi LIU
;
Mei-Liang WEI
;
- Publication Type:Journal Article
- Keywords:
Water body,Virus,Concentration methods,Optimizing experimental conditions
- From:
Microbiology
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
In this experiment six methods,calcium chloride(CaCl_(2)) precipitation,polyethylene glycol(PEG,pH7.0) precipitation,polyethylene glycol(PEG,pH11.5) precipitation,aluminum chloride(AlCl_(3)) precipitation,Amicon Utcra centrifugal filter devices and cellulose nitrate membrane were used to concentrate the vaccine poliovirus type 1(PV_(1)) added to water body;experimental conditions for concentration were selected and optimized.The results showed that two methods,CaCl_(2)and PEG(pH 7.0) precipitation were suitable for concentrating virus in large volumes of water while amicon utcra centrifugal filter devices for small ones.The virus recovery of the three methods reached a 100% rate.
