Optimum Culture Conditions for Bacterial Cellulose Produced by Acetobacter xylinum NUST4
- VernacularTitle:Acetobacter xylinum NUST4合成细菌纤维素发酵条件的优化
- Author:
Ling-Li ZHOU
;
Dong-Ping SUN
;
Qing-Hang WU
;
Shu-Lin YANG
;
- Publication Type:Journal Article
- Keywords:
Acetobacter xylinum NUST4,Bacterial cellulose
- From:
Microbiology
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
The optimum culture conditions for Acetobacter xylinum NUST4 which produces bacterial cellulose(BC) were obtained by uniform design method.BC production was dependent on MgSO_(4)?7H_(2)O,FeSO_(4)?7H_(2)O and cosubstrates such as p-aminobenzoic acid,nicotinamide,d-Biotin and ethanol.The optimal medium contained glucose 24g,sucrose 22g,peptone 16g,HAc 2.4mL,Na_(2)HPO_(4)?12H_(2)O 3.5 g,KH_(2)PO_(4)?H_(2)O 1 g,MgSO_(4)?7H_(2)O 6 g,FeSO_(4)?7H_(2)O 0.015g,nicotinamide 0.003 g,d-Biotin 0.02g and ethanol 20mL in 1L culture medium.BC yield reached 9.87g(the dried weight) in stationary culture for 7 days,which was 12-fold higher than that in the S-H medium.
