Effect of Temperature on Lycopene Prodmction by Recombinant E.coli and Control Strategy
- VernacularTitle:温度对重组大肠杆菌产番茄红素的影响及控制策略
- Author:
Xiang ZOU
;
Zhi-Hua LIAO
;
- Publication Type:Journal Article
- Keywords:
Recombinant E.coli, Lycopene, Batch fermentation, Stra tegy of temperature control
- From:
Microbiology
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Batch fermentation of lycopene by recombinant E. c ol i ZYL-2 at various temperatures ranging between 23℃～37℃ were studied in 7L fermentor. At 33℃, cell specific growth rate in earlier stage of culture was higher, and the time obtained maximum cell dry weight was shorter at other tempe rature. while after 9 h, lycopene specific production rate was higher at 28℃. Based on these results, a two-stage temperature control strategy was developed in which 33℃ was used for fermentation for the first 9 h and the temperaturew as switched to 28℃ after 9h. Using this temperature-shift strategy, the maxim al lycopene content and productivity reached 605.25?g L-1 and 28.82?g L-1h-1. The lycopene fermentation level obtained by the strategy was higher than those in single temperature-control experiments.
