Study on the Fermentation Condition of Steroid 15?-Hydroxylation by Penicillium raistrickii
- VernacularTitle:Penicillium raistrickii15?羟基化左旋乙基甾烯双酮工艺研究
- Author:
Jun LI
;
Xu GENG
;
Liang WENG
;
Zi-Rong WU
;
- Publication Type:Journal Article
- Keywords:
Penicillium raistrickii,15?-Hydroxylation, 18-methyl- estr-4-ene-3,17-dione
- From:
Microbiology
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
The fermentation conditions which affect C-15? hydroxylation o f 18-methyl-estr- 4-ene-3,17-dione were investigated. As the key step in the Hydroxylation, the dissolution of substrate was focused on. Tween80, MeOH, DMSO,?-CD and 2-HP?CD were studied to improve the dissolvability of 18-met hyl-estr-4-ene-3,17-dione.The other factors such as pH, substrate concent ration and aeration strategies which affected conversion rate were also resea rched. As a result, the conversion rate can be up to 60% in shake flask and ach i(eve 50% in fermentor,which would overcome the disadvantage of 15?-hydroxyl -18-methyl-estr-4-ene-3,17-dione chemosynthesis and provide a good techn ics to industry.
