Screening and Identification of ?-PL Producing Strain
- VernacularTitle:?-聚赖氨酸生产菌株的筛选和鉴定
- Author:
Hong-Yang ZHU
;
Hong XU
;
Qun WU
;
Wei-Wei CHEN
;
- Publication Type:Journal Article
- Keywords:
Screening, Identification,?-polylysine, Kitasatospora, 1 6S rDNA
- From:
Microbiology
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
A simple and sensitive method was developed to screen ?-PL p ro duced strains from soils. 150mg/L K_2Cr_2O_7 was added to th e solid medium for actinomycetes enriching. A basic dye, methylene blue, incorp orated in the agar plate to detect alkali producers, because dye reacted with th e secreted basic polymers by electrostatic interaction with the secreted basic p dymers and formed special zoon. And then dragendorff regent was used to detect alkaloid producers. Four ?-PL producers were obtained by TLC analysis. Meanw hile, phylogenetic analysis of PL6-3 strain, including morphology, physiologica l and biochemical characters and chemotaxomy were performed and phylogenetic tre e was constructed based on the 16S rDNA sequences. And the results indicated th at PL6-3 strain is a member of Kitasatospora.
