Effects of Psychological Stress on Peripheral Blood Lymphocyte Apoptosis and Bax,Bcl-2 Gene Expression in Rats
- VernacularTitle:心理应激对大鼠淋巴细胞凋亡及基因调控的影响
- Author:
Jun YAN
;
Ai-Guo CHEN
;
- Publication Type:Journal Article
- Keywords:
stress;
apoptosis;
gene regulation;
animal study;
lymphocytes
- From:
Chinese Mental Health Journal
2002;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of the psychological stress on peripheral blood lymphocytes apoptosis and Bax,Bcl-2 gene expression in rats after being given different intensity of noise stress.Methods:All 24 SD rats were divided into three groups:control,high intensity of the noise stress,middle intensity of the noise stress. The apoptosis in peripheral blood lymphocytes(PBL)of rats was demonstrated with the flow cytometer to determine the percentages of apoptosis in rat PBL,and the expression changes of related Bcl-2,Bax genes were observed by us- ing immunohistochemical method.Results:1)After high intensity of the noise stress for three weeks,the apoptosis in rat PBL increased significantly compared with the control.2)After high intensity of the noise stress for three weeks, Bcl-2 expression as down-regulation in PBL,but the B ax expression remained obviously high.Conclusion:It indi- cates that apoptosis exists in the immunocytes of rats in the course of high intensity of the psychological stress and it consists with the expression of the related regulatory genes.
