Recombinant Design of Fc Fusion Protein of Human ScFv Against BoNTa
- VernacularTitle:抗A型肉毒毒素人源单链抗体融合蛋白的重组设计
- Author:
Hui WANG
;
Jing SHI
;
Lu-Lu MENG
;
Pei-Zhen LI
;
Yu BAI
;
Jun YIN
;
- Publication Type:Journal Article
- Keywords:
Botulinum neurotoxin serotype A, ScFv fusion protein, Relative affinity, Stability
- From:
Microbiology
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Human ScFv against botulinum neurotoxin serotype A(BoNTa) was modified by fusing human IgG1 Fc to C terminal of ScFv. ScFv-Fc fusion protein was expressed at high level over 30% of total host cell proteins in E.coli. Recombinant protein existed in inclusion body form. Renatured ScFv-Fc was purified to 90%~95% by Protein G Sepharose column. In vitro ScFv-Fc could bind specific to toxiod BoNTa in ELISA. Recombinant ScFv-Fc had similar relative affinity to parent ScFv and had improved stability.
