Study on Glycolic Acid Oxidase (GO) of Spirulina(Arthrospira)
- VernacularTitle:螺旋藻乙醇酸氧化酶(GO)的研究
- Author:
Zhi-Zhong WANG
;
Dong-Hui GONG
;
Hua LIU
;
Chen QIAO
;
- Publication Type:Journal Article
- Keywords:
Spirulina(Arthrospira), Glycolic acid oxidase (GO), Enzyme kinetics, Alkaline lake in Erdos plateau
- From:
Microbiology
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
A comparative study on the glycolic acid oxidase (GO) of the domestic Spirulina(Arthrospira) platensis (S_(1)) from alkaline lake in Erdos Plateau and the imported S. (A.) platensis (S_(2)) and S. (A.) maxima (S_(3)) as well is made with colorimetric method. The results show that activity of GO (25℃, pH 8.0) of S_(1), S_(2 )and S_(3) is 70.9 U/gFW, 59.6 U/gFW and 80.9 U/gFW respectively; the GO's optimum temperature of S_(1)、S_(2) and S_(3 )is 30℃; the GO's optimum pH value of S_(1 )is 8.6,while that of S_(2 ) 8.2 and that of S_(3) 8.4; the GO of S_(1 )is stable from 0℃ to 35℃ and from pH 7.6 to pH 10.0, while that of S_(2) from 0℃ to 30℃ and from pH 8.0 to pH 9.0 and that of S_(3) from 0℃ to 35℃ and from pH 8.0 to pH 8.6. Adaptive range of S_(1) GO for temperature and pH is wider, and activity at low and high temperature and under strong acidand alkali conditions is higher than that of the imported species.