Optimization of Bacteriocin Produced by Lactobacillus pentosus 31-1
- VernacularTitle:戊糖乳杆菌31-1菌株产细菌素发酵条件优化
- Author:
Yan-Ni LV
;
Ping-Lan LI
;
- Publication Type:Journal Article
- Keywords:
Lactobacillus pentosus, Bacteriocin, Optimization
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
To optimize bacteriocin production,researching was done on the respect of incubation condition and the media components. And the optimum incubation time was 24h, the optimum temperature was 30℃, the optimum broth initial pH was 6.5. The optimum media component was: lactose 30g, trypone 15g, soybean peptone 20g, meat extract 30g, peptonethe 20g, Tween 80 1mL, K_(2)HPO_(4 )2g, NaAc 5g, Tri-Ammonium citrate 2g, (MgSO_(4)) 0.58g, MnSO_(4) 0.25g, H_(2)O 1000mL. Production of bactericoin under the optimum broth was 640AU/mL, 8 times than MRS media.
