Isolation, Identification and 16S rDNA Sequences Analysis of a Bacterial Resistant to Copper and Cadmium
- VernacularTitle:一株抗重金属铜镉细菌的分离、鉴定及其16S rDNA的序列分析
- Author:
Yuan-Yuan PAN
;
Wen-Li CHEN
;
Qiao-Yun HUANG
;
- Publication Type:Journal Article
- Keywords:
Heavy metals, Enterobacteraerogenes, 16S rDNA, MICs[HT]
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
A strain of resistance to copper and cadmium with high concentration, named NTG-01, was isolated from soils of DaYe county mineral area in HuBei province. It can resist copper of 4.5mmol/L and cadmium of 2mmol/L , so we can say that it is a important strain used to study the resistance mechanism of copper and cadmium. A series of morphological and biochemical characteristics and sequences analysis of 16S rDNA reveal that it belongs to the bacteria and is gram negative, short rod, flagella around, the size of bacteria is about 0.8?m?2.0?m , V-P result shows positive, methyl-red result displays negative, and glucose can be utilized to produce acid and gas; In addition to, we find that it has the percent 99 homologous to Enterobacteraerogenes by 16S rDNA sequences BLAST analysis, plus the results of morphological and biochemical parameters, it belongs to Enterobacteraerogenes. We can conclude that NTG-01 has higher resistance to many different heavy metals by measuring MICs values of nine heavy metals at last.
