The Selection of Thrombin-specific scAbs by Alternative Panning Strategy
- VernacularTitle:轮换淘选法筛选凝血酶特异的噬菌体抗体
- Author:
Long HONG
;
Bai-Cheng YANG
;
Sheng-Geng ZHU
;
- Publication Type:Journal Article
- Keywords:
Alternative panning strategy, Phage antibody library, Thrombin-specific scAb
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Specific scAbs could be obtained through biopanning from phage antibody libraries by use of antigens as target molecules. scAbs specific to thrombin were separated from mouse antibody library by the panning strategy of alternating liquid-solid phase in this paper. Thrombin was biotinylated by photobiotin at first, then avidin-coated magnetic beads were utilized to isolate specific scAbs. The eluted phages were amplified and subject to the second round panning in microtiter plate to remove the unspecific reombinant phages. 4 specific scAbs were separated from 23 phage clones after four rounds of alternative panning.
