The acquisition of recombinant pigment epithelium-derived factor and the effect on the angiogenesis of gliomas
- VernacularTitle:重组色素上皮细胞衍生因子的制备及其对脑胶质瘤血管新生的影响
- Author:
Tao ZHANG
;
Ming GUAN
;
Yuan LV
;
- Publication Type:Journal Article
- Keywords:
Glioma;
Angiogenetic factor;
Pigment epithelium-derived factor
- From:
Chinese Journal of Laboratory Medicine
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To express the pigment epithelium-derived factor (PEDF) in E.coli. and to assess its role in angiogenesis of gliomas.Methods The expression plasmid pET41/PEDF was constructed and the protein was purified by metal affinity tag (His Tag ) and anion-exchange chromatography. Tube formation was used for evaluating its biological activity. The changes of vascular enthothelial growth factor (VEGF) and thrombospondin-1 (TSP-1) were evaluated by the enzyme-linked immunosorbent assay.Results The high purified and inhibitory-actived PEDF proteins were acquired and PEDF had the function of decreasing the VEGF 1.8 times as well as increasing the TSP-1 5.3 times.Conclusions It is possible that PEDF expression is a potent factor for the inhibition angiogenesis in gliomas.
