Breeding Selenium-Enriched Yeast by Protoplast Mutagenesis.
- VernacularTitle:利用原生质体诱变育种选育富硒能力强的酵母菌株
- Author:
Yu-Rong GAO
;
Da-Peng LI
;
- Publication Type:Journal Article
- Keywords:
Protoplast,Mutate,Breed,Selenium-enriched
- From:
Microbiology
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
This paper studied on breeding selenium-enriched yeast by protoplast mutagenesis. A strain which the content of selenium is the highest is selected from thirteen strains yeast. The optimum conditions to form protoplast are lysed by 1 g/100 mL lywallzyme for 120 min, the formation and regeneration being 95.2% and 21.8% respectively. By mutating breed a strain of A1 which the content of selenium is 821 mg/kg and the amount of dry cell of 0.84 g/100 mL is obtained.
