Screening of Mutants of Carbofuran Degrading Bacterium CFDS-1 by Transposon Tagging
- VernacularTitle:转座子标签法突变呋喃丹降解菌CFDS-1
- Author:
Jian-Hong XU
;
Qing HONG
;
Ting WANG
;
Xiao-Hua ZHANG
;
Shun-Peng LI
;
- Publication Type:Journal Article
- Keywords:
Carbofuran,Transposon tagging,Degrading,Mutate
- From:
Microbiology
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to mutate a carbofuran degrading strain CFDS-1,transposon of pSC123 was introduced into the genomic DNA of strain CFDS-1 by conjugation with E.coli DH5? (pSC123) as donor and strain CFDS-1 as recipient,6 mutants which lost carbofuran degrading ability were obtained with a kanamycin resistant gene in the middle of transposon and the disappearance of a red compound during the degrading of carbofuran as preliminary selecting marks,they were designated as CFDS-M1～CFDS-M6. UV scanning and GS assay results also proved their mutations. PCR was carried out respectively with primers designed according to the sequence of transposon and genomic DNA of 6 mutants as templates,restriction analysis of PCR products showed that the mutation of carbofuran degrading genes of these mutants was caused by transposon insertion.
