Optimization of Conditions on Submerged Fermentation of Bacillus cereus
- VernacularTitle:蜡质芽孢杆菌DLSL-2发酵条件探讨及培养基优化
- Author:
Ye LI
;
Xiao-Ping ZHANG
;
Ke-Qiang ZHANG
;
Jun-Xing LI
;
Wen-Jun SUN
;
- Publication Type:Journal Article
- Keywords:
Bacillus cereus, Fermentation, Medium optimization , Uniform design
- From:
Microbiology
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
The fermentation of Bacillus cereus DLSL-2 was investigated through single-factor test. The optimized fermentation conditions are: temperature 30℃,initial pH 7.0,250 r/min. Uniform design was used in shaking flask to optimize the fermentation medium of bacillus cereus . The most suitable medium was identified as follows:4.88% defated soybean power ,1.45% maize starch, 0.106% K 2HPO 4, 1.78% yeasts extract, 5.6% inoculum. the optimized medium allowed the B.cereus biomass concentration to be increased from 3.2?109 cfu/mL to 7.1?109 cfu/mL.
