Study on the Recombinant Human ApoA-I_Milano High Density and High Expression by Two Temperature-Shifted Induction in Escherichia coli
- VernacularTitle:两次诱导实现重组大肠杆菌高密度、高表达研究
- Author:
Wen-Feng MA
;
Ying-Ping ZHUANG
;
Mei-Jin GUO
;
Man-Sheng DING
;
Ju CHU
;
Si-Liang ZHANG
;
- Publication Type:Journal Article
- Keywords:
Recombinant E.coli,ApoA-IMilano,High density,High expression,Two temperature-shifted induction
- From:
Microbiology
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
The temperature effect on the recombinant protein production formation was investigated in present study. The culture temperature of growth phase is 30℃, and the culture temperature of induction phase was arranged according to three modes. Hign cell-density and high expression culture of E.coli to product recombinant human apolipoprotein A-I Milano by two temperature-shifted induction . Two temperature-shifted induction was carried out high density and high expression recombinant human ApoA-1 Milano. The recombinant protein ApoA-I Milano reached 4.8 g?L -1 with the final cell density of OD 600 150. And the two temperature-shifted induction avoided the acetic acid successfully to the influence of the high density and high expression. Two temperature-shifted induction was viable in high density culture and high expression of heterogenous protein in recombination E.coli.The sduty provides a basic work for production of recombinant ApoA-I Milano in scale.
