Enhance the Expression of B. subtillis Fibrinolysis Enzyme by degQ Gene
- VernacularTitle:利用degQ基因提高枯草芽孢杆菌纤溶酶表达
- Author:
Ming-Fei JIN
;
Xin-Hua ZHU
;
Li JIN
;
Hui-Fang BIAN
;
Zi-Rong WU
;
- Publication Type:Journal Article
- Keywords:
DegQ gene, Enhanced expressing, Induced expressing, Bacillus subtillis
- From:
Microbiology
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
The degQ gene, amplified from Bacillus subtilis by PCR, was cloned to pUBS (sucrose induced secretion vector). After transformed into DB403, recombination named DB403(pUBSD) was formed. The results of the fermentation showed that degQ gene enhanced the expression of B. subtilis fibrin enzyme. The activity of the enzyme was increased to 2.2 times as the original one. In this article, the effects of different conditions, such as different kinds of sugar, different concentration of sucrose and different induced time were also be investigated and compared.
