Construction of M1GS for Targeted Cleavage of HCMV UL54 mRNA Segments
- VernacularTitle:对HCMVUL54 mRNA片段特异性切割的M1GS构建
- Author:
Jing-Zhu LV
;
Hong-Jian LI
;
Hao-Jun CHEN
;
Yue-Qin LI
;
Tian-Hong ZHOU
;
- Publication Type:Journal Article
- Keywords:
Human cytomegalovirus(HCMV),UL54,RNase P,External guide sequences (EGSs)
- From:
Microbiology
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Human cytomegalovirus (HCMV) is a DNA virus and serious opportunistic pathogen for both newborn and immunocompromised individuals.To research technique for gene silence and antiviral agents, ribozyme M1GS-T6 was constructed from external guide sequences(EGSs)that consist of a sequence complementary to HCMV UL54 gene RNA and M1 RNA, the catalytic RNA subunit of RNase P from Escherichia coli. The results showed that M1GS can efficiently cleave the mRNA sequence encoding UL54 protein in vitro.
