Evaluation of Vitek and Disk Diffusion Susceptibility Testing of Enterobacteriaceae against Piperacillin-Tazobactam and Methicillin-Resistant Staphylococcus aureus against Trimethoprim-Sulfamethoxazole.
- Author:
Wonkeun SONG
1
;
Tae Jae LEE
;
Taek Kyung KIM
;
Han Sung KIM
;
Jae Seok KIM
;
Min Jeong PARK
;
Kyu Man LEE
Author Information
1. Department of Laboratory Medicine, Hallym University College of Medicine, Seoul, Korea. swonkeun@hallym.or.kr
- Publication Type:Original Article
- Keywords:
Enterobacteriaceae;
Methicillin-resistant Staphylococcus aureus;
Piperacillintazobactam;
Trimethoprim-sulfamethoxazole;
Vitek;
Disk diffusion
- MeSH:
Citrobacter freundii;
Diffusion*;
Enterobacter cloacae;
Enterobacteriaceae*;
Escherichia coli;
Klebsiella pneumoniae;
Methicillin Resistance*;
Methicillin-Resistant Staphylococcus aureus*;
Pantoea;
Serratia marcescens;
Trimethoprim, Sulfamethoxazole Drug Combination*
- From:Korean Journal of Clinical Microbiology
2005;8(2):160-164
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The aim of this study was to evaluate the Vitek system and the disk diffusion method for susceptibility testing of Enterobacteriaceaeagainst piperacillin-tazobactam (TZP) and methicillin-resistant Staphylococcus aureus (MRSA) against trimethoprim-sulfamethoxazole (SXT)using the broth microdilution method as the reference. METHODS: Using the Vitek system and the disk diffusion method, we tested 96 isolates of Enterobacteriaceae (48 Escherichia coli, 26 Klebsiella pneumoniae, 8 Serratia marcescens, 6 Enterobacter cloacae, 2 E. aerogenes, 2 K. oxytoca, 2 Citrobacter freundii, 2 Pantoea agglomerans) and 61 isolates of MRSA for susceptibity against TZP and SXT, respectively; the broth microdilution of National Committee for Clinical Laboratory Standards was used as the reference method. RESULTS: In the susceptibility testing of Enterobacteriaceae against TZP, Vitek system yielded 10 (10%) minor errors, and the disk diffusion method one (1%) very major and 13 (14%) minor errors. For the MRSA against SXT, the rate of categorical agreement between the reference method and the Vitek or the disk diffusion method was both 100%. The rates of agreement between the reference method and the Vitek system in term of MICs (within +/-1 dilution) were 93% and 98% in the susceptibility testing of Enterobacteriaceae against TZP and MRSA against SXT, respectively. CONCLUSION: Both Vitek system and disk diffusion method showed an acceptable level of accuracy for the susceptibility test of Enterobacteriaceaeagainst TZP and MRSA against SXT.
