The Isolation of a Flocculant-Producing Bacillus and Identification of Microbial Flocculant
- VernacularTitle:一株产絮凝剂芽孢杆菌的分离及成份分析
- Author:
Xiao-Na MA
;
Ming HUI
;
Tian-Gui NIU
;
- Publication Type:Journal Article
- Keywords:
Microbial flocculant, Bacillus sp., Poly ?-glutamic acid
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
This paper mainly discussed a bacterial stain with high flocculating activity isolated from cantaloupe juice .The strain was named Bacillus sp.B_(53) based on colony morphology, physiological and biochemistry experiments. The new flocculant was purified and shown to be a homopolymer of glutamic acid by HLPC analysis and thin layer chromatography, and presumed to be Poly ?-glutamic acid(?-PGA). ?-PGA 12.48g/L was achieved in shake flask. The purified material showed a absorption peak at 212nm, and was only composed of L-Glu. The MW could be detected through SDS-PAGE, and its MW was about a molecular mass between 440kD with 669kD. This bioflocculant efficiently flocculated various organic and inorganic suspensions. It's flocculanting effect on kaolin and ([BF]Ca(OH)_2[BFQ]) was superior to others.
