Isolation and Identification of a Lipopeptide
- VernacularTitle:脂肽的分离纯化与结构研究
- Author:
Ying-Nian LV
;
Shi-Zhong YANG
;
Bo-Zhong MU
;
- Publication Type:Journal Article
- Keywords:
Bacillus subtilis, Lipopeptide, Isolation, Cyclic structure
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
A lipopeptide compound was isolated from the culture of Bacillus subtilis HSO121 by methods of acidic precipitation, solvent extract, fractional precipitation, adsorption and prepared thin-layer chromatography; and its molecular structure was determined by by ninhydrin assay and IR methods following the Amino analysis, MS-MS and ESI-MS. It shows that the isolated lipopeptide consists of two homologues with molecular mass 1,022D and 1,036D and bearing a cyclic structure with the amino acid sequence Leu-Leu-Asp-Val-Leu-Leu-Glu in the peptide chain, which indicates that the isolated lipopeptide falls into the analogs of surfactin.
