Optimation of the Fermentation Conditions for Human ApolipoproteinA-I Expression
- VernacularTitle:重组人载脂蛋白ApoA-I表达条件的优化
- Author:
Wen-Feng MA
;
Man-Sheng DING
;
Mei-Jin GUO
;
Ying-Ping ZHUANG
;
Ju CHU
;
Si-Liang ZHANG
;
- Publication Type:Journal Article
- Keywords:
Recombinant human apolipoprotein A-I,E.coli,Optimization,C/N ratio
- From:
Microbiology
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Optimization of the fermentation condition for human apolipoproteinA-I expression in recombinant Escherichia coli was investigated. The recombinant plasmid pBV220-ApoA-I was transformed respectively into different E.coli hosts such as JM109, BL21(DE3),DH5?, BMH7118,and TG1. The best host E.coli was DH5? in which the recombinant ApoA-I expression percentage was 21.2% corresponding to that in BL21(DE3) in flask shaker cultivation,while the ApoA-I expressed percentage in E.coli TG1 was 11%.Fed-batch cultivation was performed in FMG-5L fermentor,the optimum fermentation cultivation conditions were as following :optimum pH value was 7.0 in growth phase and 7.4 in the expression phase. The initial glucose concentration in batch phase was 3 g?L -1.The optimum C/N ratio was 2∶1.The recombinant ApoA-I reached about 40% of the total protein, and concentration of ApoA-I was 2.86 g?L -1.
