Application of two bacteria colony-PCR methods in the screening of phage antibody library
- VernacularTitle:两种菌落聚合酶链反应在抗体库筛选中的应用比较
- Author:
Guo-Zhu XUE
;
Ke-Feng DOU
;
Yong-Gang LV
;
Li-Bo YAO
;
- Publication Type:Journal Article
- Keywords:
PCR;
Bacteria colonies;
Phage antibody library;
Insert genes
- From:
Journal of Medical Postgraduates
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To value the application of two bacteria colony-PCR methods in the screening of phage antibody library. Methods:Five positive monoclonal bacterium were respectively suspended in either deionized water or 0.1% Triton X-100, and then boiled to be used as template in PCR. . The DNAs products of PCR were extracted and digested by two enzymes, and then determined by electrophoresis. Results:The inserted genes were detected in all the 5 clones after PCR and enzyme digestion .Conclusion:Bacteria colony-PCR can be used in screening positive recombinant colonies. The bacteria colony-PCR method with bacteria colonies suspended in deionized water is valuable in large scale positive recombinant bacterium screening.
